文献类型：期刊文献

英文题名：An Electrochemical Immunosensor Based on SPA and rGO-PEI-Ag-Nf for the Detection of Arsanilic Acid

作者：Wang, Yanwei[1];Ma, Dongdong[1];Zhang, Gaiping[1];Wang, Xuannian[2];Zhou, Jingming[1];Chen, Yumei[1];You, Xiaojuan[1];Liang, Chao[1];Qi, Yanhua[1];Li, Yuya[1];Wang, Aiping[1]

第一作者：Wang, Yanwei

通讯作者：Wang, AP[1]

机构：[1]Zhengzhou Univ, Sch Life Sci, Zhengzhou 450000, Peoples R China;[2]Xinxiang Univ, Sch Life Sci & Basic Med, Xinxiang 453003, Henan, Peoples R China

第一机构：Zhengzhou Univ, Sch Life Sci, Zhengzhou 450000, Peoples R China

通讯机构：[1]corresponding author), Zhengzhou Univ, Sch Life Sci, Zhengzhou 450000, Peoples R China.

年份：2022

卷号：27

期号：1

外文期刊名：MOLECULES

收录：;Scopus(收录号：2-s2.0-85122031041);WOS:【SCI-EXPANDED(收录号:WOS:000758380500001)】；

基金：FundingThis work was supported by the National Key Research and Development Plan of China (Nos. 2018YFC1602902) and (2019YFC1604501).

语种：英文

外文关键词：arsanilic acid; polyethyleneimine; silver nanoparticles; reduced graphene oxide; staphylococcal protein A; electrochemical immunosensor

摘要：A sensitive electrochemical immunosensor was prepared for rapid detection of ASA based on arsanilic acid (ASA) monoclonal antibody with high affinity. In the preparation of nanomaterials, polyethyleneimine (PEI) improved the stability of the solution and acted as a reducing agent to generate reduced graphene oxide (rGO) with relatively strong conductivity, thereby promoting the transfer of electrons. The dual conductivity of rGO and silver nanoparticles (AgNPs) improved the sensitivity of the sensor. The synthesis of nanomaterials were confirmed by UV-Vis spectroscopy, X-ray diffraction, transmission electron microscopy and scanning electron microscopy. In the optimal experiment conditions, the sensor could achieve the detection range of 0.50-500 ng mL(-1) and the limit of detection (LOD) of 0.38 ng mL(-1) (S/N = 3). Moreover, the sensor exhibited excellent specificity and acceptable stability, suggesting that the proposed sensor possessed a good potential in ASA detection. Thus, the as-prepared biosensor may be a potential way for detecting other antibiotics in meat and animal-derived foods.