文献类型：期刊文献

英文题名：Target binding and DNA hybridization-induced gold nanoparticle aggregation for colorimetric detection of thrombin

作者：Li, Li[1];Liang, Yu[1];Zhao, Yan[2];Chen, Zhengbo[2]

第一作者：李丽

通讯作者：Li, L[1];Chen, ZB[2]

机构：[1]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China;[2]Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

第一机构：新乡学院化学化工学院

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China;[2]corresponding author), Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China.|[110713]新乡学院化学化工学院;[11071]新乡学院;

年份：2018

卷号：262

起止页码：733-738

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20180804813455);Scopus(收录号：2-s2.0-85042075926);WOS:【SCI-EXPANDED(收录号:WOS:000427460600087)】；

基金：All authors gratefully acknowledge the financial support of Xinxiang Municipal Science and Technology Bureau (Grant No. ZG13012).

语种：英文

外文关键词：Thrombin; Gold nanoparticles; Colorimetric; DNA hybridization; G-quadruplex

摘要：We present here a simple and sensitive sensing strategy for thrombin in buffer solution and human serum samples. The key features of this assay lie in target binding-induced DNA hybridization and the formation of gold nanoparticle (AuNP) aggregation. In the presence of thrombin, the binding of the two DNA strands to the same target triggers the hybridization between the complementary sequences of the two DNA strands. The hybridization is able to pull a few AuNPs together to form oligomers. A linear dependence between the absorbance and target thrombin concentration is obtained under optimal conditions in the range from 10 nM-5 mu M with a detection limit (LOD) of 7.5 nM estimated at the 3S(blank) level. Moreover, this method is successfully applied to complex serum samples without complicated sample pretreatment and sophisticated instruments, a dynamic range (10 nM-5 mu M) and a detection limit as low as 9.1 nM is achieved. (C) 2018 Elsevier B.V. All rights reserved.