文献类型：期刊文献

中文题名：氧及活性氧代谢相关基因在大鼠肝脏再生中的作用

英文题名：Actions of the Genes Associated with Oxygen and Reactive Oxygen Species Metabolism During Rat Liver Regeneration

作者：蔺芳[1];张家洋[1];皮川真[2];徐存拴[3]

第一作者：蔺芳

机构：[1]新乡学院生命科学与技术系;[2]华兰生物工程股份有限公司;[3]河南省-科技部共建细胞分化调控重点实验室

第一机构：新乡学院生命科学技术学院

年份：2010

卷号：31

期号：10

起止页码：32-37

中文期刊名：动物医学进展

外文期刊名：Progress in Veterinary Medicine

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家重点基础研究发展计划(973)项目(2006CB708506)

语种：中文

中文关键词：部分肝切除;Rat;Genome2302.0芯片;氧及活性氧代谢;肝再生相关基因

外文关键词：partial hepatectomy; Rat genome 230 2.0 array; oxygen and reactive oxygen species metabolism; genes associated with liver regeneration

摘要：为了在基因转录水平了解氧及活性氧代谢相关基因在大鼠肝脏再生(LR)中的作用,用Rat Genome 230 2.0芯片检测了上述在大鼠再生肝中表达变化,用真、假手术比较方法确定肝再生相关基因。初步证实上述基因中81个与肝再生相关。其中,肝再生启动、G0/G1过渡、细胞增殖、细胞分化和组织结构功能重建等四个阶段起始表达的基因数为42、9、35和2;基因的总表达次数为42、31、77和50。表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共上调317次,下调161次,分为6类表达方式,表明肝再生中这些基因的表达变化多样和复杂。芯片结果分析表明,肝再生早期和前期过氧化氢和超氧化物代谢增强;几乎在整个肝再生中氧化反应和谷胱甘肽结合反应增强。
To study the actions of the genes associated with oxygen and reactive oxygen species metabolism during liver regeneration（LR） at transcriptional level,The genes were obtained by collecting the data of databases and referring to theses,and the gene expression changes in the rat regenerating liver were checked by the Rat Genome 230 2.0 array,and LR-associated genes were identified by comparing gene expression difference between partial hepatectomy（PH） and sham operation（SO） groups.81 genes involved in oxygen and reactive oxygen species metabolism were proved to be LR-associated.The initial and total expressing gene numbers in initiation phase of liver regeneration（0.5 h-4 h after PH）,G0/G1 transition（4 h-6 h after PH）,cell proliferation（6 h-66 h after PH）,cell differentiation and structure-function reconstruction（66 h-168 h after PH） were 42,9,35,2 and 42,31,77,50,respectively,illustrating that the associated genes mainly were triggered in initiation phase of liver regeneration（LR）,and worked in different phases.The total times of their up-and down-expression were 317 and 161 respectively,and they were classified into 6 types indicating that the activities mentioned above were diverse and complicated.The results of chip detection confirmed that the genes associated with hydrogen peroxide metabolism and superoxide metabolism were enhanced in the early phase and prophase of LR;oxidative reaction and glutathione conjugation reaction were enhanced almost in whole LR.