文献类型：期刊文献

英文题名：Electrochemical aptamer biosensor for DNA detection based on label-free aptamers

作者：Li, Li[1];Chen, Zhengbo[2]

第一作者：李丽

通讯作者：Li, L[1]

机构：[1]Xinxiang Univ, Sch Chem & Mat Engn, Xinxiang 453003, Peoples R China;[2]Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

第一机构：新乡学院

通讯机构：[1]corresponding author), Xinxiang Univ, Sch Chem & Mat Engn, Xinxiang 453003, Peoples R China.|[11071]新乡学院;

年份：2023

卷号：153

外文期刊名：BIOELECTROCHEMISTRY

收录：;EI(收录号：20232714330898);Scopus(收录号：2-s2.0-85163478549);WOS:【SCI-EXPANDED(收录号:WOS:001034109500001)】；

基金：Acknowledgements All authors gratefully acknowledge the Key Scientific andTechnological Project of Henan Province (Grant No. 222102310380 and No.222102320275) .

语种：英文

外文关键词：Triblock DNA; Aptamer biosensor; Electrochemistry; Signal amplification

摘要：Electrochemical aptasensor has been broadly advanced for nucleic acid detection. However, it is a long-term goal to design an aptasensor with high specificity, flexibility, and simplicity. In this work, we develop a strategy of triblock DNA probe, which consists of two DNA probes at both ends and ployA fragments in the middle as probe-polyA-probe. PolyA fragment has high affinity to the surface of gold electrode, so it can be assembled on the electrode surface via polyA instead of traditional Au-S bonds. When the target DNA is simultaneously hybridized with the two capture probes, the hybridization stability can be improved due to the strong base stacking effect. [Ru(NH3)6]3+, as signal probe, can be electrostatically adsorbed on the negatively charged DNA skeleton. A wide linear range (10 pM-10 & mu;M) is obtained with a detection limit of 2.9 pM. Our electrochemical aptasensor has good repeatability, stability, and specificity. More importantly, the electrochemical sensor can successfully detect DNA in human serum samples, which proves its practical value and extensive applicability in complex environment.