文献类型：期刊文献

中文题名：达氟沙星多克隆抗体制备及间接竞争ELISA方法的建立

英文题名：Preparation of Polyclonal Antibodies against Darfloxacin and Establishment of ic-ELISA Method

作者：张欣欣[1,2];齐永华[1];岳锋[1];路少鹏[1];李文明[1,2];郭东光[1];潘鹏涛[1];王选年[1]

第一作者：张欣欣

机构：[1]新乡学院动物疫病分子诊断河南省工程实验室,河南新乡453000;[2]郑州大学,河南郑州450000

第一机构：新乡学院

年份：2022

卷号：43

期号：12

起止页码：84-89

中文期刊名：动物医学进展

外文期刊名：Progress In Veterinary Medicine

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：国家重点研发计划项目(2018YFC1602900)。

语种：中文

中文关键词：达氟沙星;完全抗原;多克隆抗体;间接竞争酶联免疫吸附试验

外文关键词：danofloxacin;complete antigen;polyclonal antibody;ic-ELISA

摘要：为制备达氟沙星(DAN)多克隆抗体,建立检测DAN残留的间接竞争ELISA(ic-ELISA)方法,采用混合酸酐法制备免疫原DAN-BSA和包被原DAN-OVA,用DAN-BSA免疫新西兰大白兔获得DAN多抗血清,建立检测DAN的ic-ELISA检测方法。结果显示,成功合成了DAN-BSA和DAN-OVA,并获得DAN的多克隆抗体;用该抗体建立的ic-ELISA检测方法的半数抑制浓度为0.39μg/L,最低检测限为0.04μg/L,检测范围为0.09μg/L~6.16μg/L。结果表明,成功制备了抗DAN的多克隆抗体,并建立了检测DAN的ic-ELISA方法,为进一步开发DAN快速检测试剂盒奠定了基础。
To prepare danofloxacin(DAN)polyclonal antibodies and establish an ic-ELISA method for detecting DAN residue,the immunogen DAN-BSA and the coating original DAN-OVA were prepared by the mixed acid anhydride method.The polyclonal antibodies were obtained by immunizing New Zealand white rabbits with DAN-BSA,and an ic-ELISA to detect DAN was established.The results showed that DAN-BSA and DAN-OVA were successfully synthesized,and DAN polyclonal antibodies were successfully obtained;The half inhibitory concentration of the ic-ELISA method is 0.39μg/L,the lowest detection limit is 0.04μg/L,and the detection range is 0.09μg/L-6.16μg/L.In summary,the ic-ELISA method was established with polyclonal antibodies for detecting DAN,which laid the foundation for the further development of DAN rapid detection kit.