文献类型：期刊文献

中文题名：大豆SBP基因家族的序列特征、表达及进化分析

英文题名：Sequence characterization,expression pattern,and evolutionary analysis of SBP-domain gene family in soybean(Glycine max)

作者：朱红霞[1];胡利宗[2,3];邓小莉[1];蔺芳[1]

第一作者：朱红霞

机构：[1]新乡学院生命科学与技术系;[2]中国科学院遗传发育所;[3]中国科学院研究生院

第一机构：新乡学院生命科学技术学院

年份：2012

卷号：43

期号：7

起止页码：26-33

中文期刊名：东北农业大学学报

外文期刊名：Journal of Northeast Agricultural University

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：河南省政府决策研究招标课题(B578)

语种：中文

中文关键词：大豆;结构域;基因家族;进化

外文关键词：soybean; domain; gene family; evolution

摘要：SBP基因家族是植物所特有的一类重要转录因子,由多个成员组成,主要参与植物生长、发育以及多种生理生化过程。试验在大豆基因组中鉴定49个SBP基因,被命名为GmSBP1~49。基于生物信息学手段,对大豆该基因家族49个成员的基因结构、染色体定位、蛋白保守序列、亚细胞定位、表达情况及进化关系进行分析。序列分析表明,SBP基因家族成员分散于不同染色体上,不同基因具有不同个数的外显子,其数目变异范围为1~14;该家族蛋白含有5个保守基序,尽管与SBP结构域有所重叠,但它们能形成6种不同的组织模式,这说明该基因家族序列变异较为复杂。表达分析结果显示,除GmSBP2和GmSBP11等6个基因没有相应的EST外,其余基因都有转录活性;在具有转录活性的基因中,只有GmSBP46显示出组成型表达模式,剩余基因表现出不同程度的组织特异性表达模式。拟南芥、水稻和大豆SBP蛋白的进化树揭示该家族具有8个类群,其中E类群只包括大豆SBP基因,其他类群中大豆SBP基因数目也是最多,这充分说明大豆SBP基因家族起源与进化的复杂性。研究为大豆SBP基因功能研究提供线索。
The multiple members of a SBP-domain gene family,which are transcript factors specific to plants,mainly involve in growth,development,and various physiological and biochemical processes in plants.In this study,49 members of SBP-domain gene family were identified in soybean genome,and named as GmSBP1-49,respectively.A systematic analyses that contained gene structure,chromosomal mapping,protein conserved motifs,subcellular position,expression pattern,and phylogenetic relationships of all the members of SBP-domain gene family were performed using bioinformatics approach.The results of sequence feature analysis showed that the members of a SBP-domain gene family distributed on almost all the chromosomes;different members had different numbers of exons,ranging from 1 to 14;five conserved motifs shared the same region with SBP domain in SBP proteins,but there were six different organization patterns of five conserved motifs.This suggests that sequence variation is much complex in this gene family.In addition,expression pattern analysis indicated that most of SBP genes were likely to be transcriptional active,except from six SBP genes with no activity of transcription including GmSBP2,GmSBP11,and so on.Interesting,GmSBP46 was constitutively expressed in all the examined tissues,whereas expression patterns of the remaining genes were specifical in certain tissues.Phylogenetic relationships of SBP-domain proteins of Arabidopsis thaliana,Oryza sativa,and Glycine max showed that there were eight major groups.E group only contained two soybean SBP genes.Additionally,there were more soybean SBP genes than Arabidopsis and rice in other groups.The results showed that a complex mechanism had affected the origin and evolution of the soybean SBP gene family.This study provides useful clues for further investigation of SBP-domain gene function in soybean（Glycine max）.