文献类型：期刊文献

英文题名：Quantum dots and p-phenylenediamine based method for the sensitive determination of glucose

作者：Lv, Xiaoxiao[1];Wang, Xiaoyu[1,2];Huang, Dawei[1];Niu, Chenggang[1];Zeng, Guangming[1];Niu, Qiuya[1]

第一作者：Lv, Xiaoxiao

通讯作者：Niu, CG[1]

机构：[1]Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China;[2]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China

第一机构：Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China

通讯机构：[1]corresponding author), Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.

年份：2014

卷号：129

起止页码：20-25

外文期刊名：TALANTA

收录：;EI(收录号：20142417819398);Scopus(收录号：2-s2.0-84901976129);WOS:【SCI-EXPANDED(收录号:WOS:000341463400004)】；

基金：This work was financially supported by the National Natural Science Foundation of China (20977026 and 51108166), the National 863 High Technology Research Foundation of China (2006AA06Z407), the Hunan Provincial Natural Science Foundation of China (14JJ2045), and a Project supported by Scientific Research Fund of Hunan Provincial Education Department (13k017).

语种：英文

外文关键词：Glucose; p-phenylenediamine; Quantum dots; Time-gated fluorescence; Glucose oxidase

摘要：By introducing p-phenylenediamine (PPD) to the hybrid system of Mn-doped CdS/ZnS quantum dots (QDs) and glucose oxidase (GOD), a sensitive label-free method was proposed for direct detection of glucose. With glucose and PPD as substrates, 2,5-diamino-N,N'-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine (DDACD) that intensively quenches the fluorescence of QDs can be produced by the catalysis of GOD. A detection limit as low as 3.2 mu M was obtained with the high-efficient fluorescence quencher. Two linear ranges, from 5.0 mu M to 1000 mu M and from 1.0 mu M to 10.0 mu M, were identified between time-gated fluorescence intensity and the concentration of glucose. It is shown that the newly proposed methods have high selectivity for glucose over other saccharides and coexisting biological species in serum. The method can be used directly to determine glucose in normal adult human serum without any complicated sample pretreatments. The recovery rate and repeatability of the method were also shown to be satisfactory. (C) 2014 Elsevier B.V. All rights reserved.