登录    注册    忘记密码

详细信息

A Fluorescence Sensor for Lead (II) Ions Determination Based on Label-Free Gold Nanoparticles (GNPs)-DNAzyme Using Time-Gated Mode in Aqueous Solution  ( SCI-EXPANDED收录 EI收录)  

文献类型:期刊文献

英文题名:A Fluorescence Sensor for Lead (II) Ions Determination Based on Label-Free Gold Nanoparticles (GNPs)-DNAzyme Using Time-Gated Mode in Aqueous Solution

作者:Wang, Xiao-Yu[1,2];Niu, Cheng-Gang[1];Guo, Li-Juan[1];Hu, Liu-Yin[1];Wu, Sheng-Quan[1];Zeng, Guang-Ming[1];Li, Fei[1]

第一作者:王晓钰;Wang, Xiao-Yu

通讯作者:Niu, CG[1]

机构:[1]Hunan Univ, Coll Environm Sci & Engn, Key Lab Environm Biol & Pollut Control, Minist Educ, Changsha 410082, Hunan, Peoples R China;[2]Xinxiang Univ, Coll Chem & Chem Engn, Xinxiang 453003, Peoples R China

第一机构:Hunan Univ, Coll Environm Sci & Engn, Key Lab Environm Biol & Pollut Control, Minist Educ, Changsha 410082, Hunan, Peoples R China

通讯机构:[1]corresponding author), Hunan Univ, Coll Environm Sci & Engn, Key Lab Environm Biol & Pollut Control, Minist Educ, Changsha 410082, Hunan, Peoples R China.

年份:2017

卷号:27

期号:2

起止页码:643-649

外文期刊名:JOURNAL OF FLUORESCENCE

收录:;EI(收录号:20165003108562);Scopus(收录号:2-s2.0-85000910723);WOS:【SCI-EXPANDED(收录号:WOS:000395070900028)】;

基金:This work was financially supported by the National Natural Science Foundation of China (51541801, 51521006), the Hunan Provincial Natural Science Foundation of China (14JJ2045), and a Project Supported by Scientific Research Fund of Hunan Provincial Education Department (13 k017).

语种:英文

外文关键词:Time-gated fluorescence; DNAzyme; Gold nanoparticles; Terbium ternary complex

摘要:This paper describes a label-free 17E DNAzyme-based time-gated fluorescence sensor for Pb2+ detection by unmodified gold nanoparticles (GNPs) and a terbium ternary complex. The fluorophore that used in this paper is a terbium ternary complex. Its signal can be measured in a time-gated manner which could eliminate most of the unspecific fluorescent background. It is well known that unfolded single-stranded DNA (ssDNA) could be adsorbed on GNPs while double-stranded DNA could not. The cleavage of the substrate by the 17E DNAzyme in the presence of Pb2+ causes the release of ssDNA from the 17E-17S duplex to be absorbed onto GNPs, preventing the aggregation of GNPs and then leading to a fluorescence decrease of terbium ternary complex. By means of this method, the authors have successfully detected Pb2+ over a range of 10 nM to 2500 nM with a detection limit of 1.7 nM. The sensor also exhibited good selectivity. The sensor provided a simple, cost-effective, rapid and sensitive measurement tool for Pb2+ detection.

参考文献:

正在载入数据...

版权所有©新乡学院 重庆维普资讯有限公司 渝B2-20050021-8 
渝公网安备 50019002500408号 违法和不良信息举报中心