文献类型：期刊文献

英文题名：Identification of the linear ligand epitope on classical swine fever virus that interacts with porcine kidney 15 cells

作者：Mei, Yin[1];Yue, Feng[2];Ning, Hong-mei[1];Zhou, Juan-juan[2];Wang, Xuan-nian[2]

第一作者：Mei, Yin

通讯作者：Wang, XN[1]

机构：[1]Henan Inst Sci & Technol, Coll Vet & Anim Sci, Xinxiang 453003, Henan, Peoples R China;[2]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China

第一机构：Henan Inst Sci & Technol, Coll Vet & Anim Sci, Xinxiang 453003, Henan, Peoples R China

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2017

卷号：81

期号：3

起止页码：186-191

外文期刊名：CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE

收录：;Scopus(收录号：2-s2.0-85024129745);WOS:【SCI-EXPANDED(收录号:WOS:000408439200004)】；

基金：This work was supported by the National Natural Science Foundation of China (grant 31201877) and the Foundation of the Henan Educational Committee (grant 16A230001).

语种：英文

摘要：Binding of the viral ligand to a specific receptor is the first step of virus entry into target cells. The envelope proteins Erns, E1, and E2 of classical swine fever virus (CSFV) are involved in the interaction with host cell receptors to mediate CSFV infection. The aim of this investigation was to identify epitopes that bind to porcine kidney (PK)-15 cells to prevent CSFV infection. Ten peptides representing Erns, E1, and E2 were synthesized. Immunohistochemical study showed that the SE24 peptide, which is derived from the E2 amino acid sequence, could effectively bind to PK-15 cells. Similarly, a flow cytometry assay demonstrated that SE24 binding to PK-15 cells could be blocked by CSFV. The binding of SE24 with PK-15 cells leads to decreased CSFV infection of PK-15 cells in a dose-dependent manner. These results suggest a potential new strategy for the prevention and control of CSFV infection that requires further investigation.