文献类型：期刊文献

英文题名：Examination of Quaternary Ammonium Compound Resistance in Proteus mirabilis Isolated from Cooked Meat Products in China

作者：Jiang, Xiaobing[1];Yu, Tao[2];Liu, Lei[1];Li, Yi[1];Zhang, Kun[1];Wang, Hailei[1];Shi, Lei[3]

第一作者：Jiang, Xiaobing

通讯作者：Yu, T[1]

机构：[1]Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China;[2]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang, Peoples R China;[3]Jinan Univ, Inst Food Safety & Nutr, Guangzhou, Guangdong, Peoples R China

第一机构：Henan Normal Univ, Coll Life Sci, Xinxiang, Peoples R China

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Life Sci & Technol, Xinxiang, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2017

卷号：8

期号：DEC

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85037742704);WOS:【SCI-EXPANDED(收录号:WOS:000417576200001)】；

基金：This work was supported by the National Natural Science Foundation of China (31601568), the Key Project of Natural Science of the Education Department of Henan Province, China (15A180006 and 16A180011), the Key Scientific and Technological Project of Xinxiang (ZG15007) and the Doctoral Scientific Research Foundation of Henan Normal University.

语种：英文

外文关键词：Proteus mirabilis; qac genes; integron; benzalkonium chloride; resistance

摘要：The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs) and the association of qac genes with class 1 integrons in Proteus mirabilis isolated from cooked meat products. A total of 52 P. mirabilis isolates (29.2%) were detected from 178 samples, and their minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) ranged from 4 to > 32 mu g/mL. The isolates with BC MICs of 24 mg/mL were observed most frequently. PCR assays indicated that mdfA, ydgE/ydgF, qacE, qacE 1 1, emrE, sugE(c), and sugE(p) were commonly present (32.7%-100%) in these isolates, but qacH was less prevalent (3.8%). Five groups of resistance gene cassettes were identified in 10 intI1-positive isolates. An unusual gene cassette array dfrA32-ereA-aadA2 was found in one foodborne isolate of P. mirabilis. Two isolates harbored qacH-and sul3-associated non-classic integrons: aadA2-cmlA1-aadA1-qacH-IS440-sul3 and a new arrangement dfrA32-ereA1-aadA2-cmlA1-aadA1-qacH-IS440-sul3, which is first reported in P. mirabilis. Non-classic class 1 integrons were located on conjugative plasmids of 100 kb in two tested isolates. Our data showed that the QAC resistance genes were commonly present among P. mirabilis isolates from cooked meats and qacH was associated with non-classic class 1 integrons. The creation of transconjugants demonstrated that qacH-associated non-classic class 1 integrons were located on conjugative plasmids and therefore could facilitate the co-dissemination of disinfectant and antimicrobial resistance genes among bacteria, an increasing area of concern.