文献类型：期刊文献

英文题名：haracterization and functional analysis of microRNA399 in Cunninghamia lanceolata

作者：Zhu, F. R.[1];Qiu, Z. B.[2];Zhang, Y. M.[2];Zhang, X. R.[2];Wang, W. L.[2]

第一作者：朱凤荣

通讯作者：Qiu, ZB[1]

机构：[1]Xinxiang Univ, Sch Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China;[2]Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China

第一机构：新乡学院生命科学技术学院

通讯机构：[1]corresponding author), Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China.

年份：2020

卷号：64

起止页码：193-199

外文期刊名：BIOLOGIA PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000518637300001)】；

基金：This work was supported by the National Natural Science Foundation of China (31500499) and the Program for Science Technology Innovation Talents in Universities of Henan Province (16HASTIT019).

语种：英文

外文关键词：Arabidopsis thaliana; Nicotiana benthamiana; PHO2; phosphate transporters; RLM-RACE

摘要：The miR399 is a conserved microRNA (miRNA) family, and it has been characterized as an essential regulator of phosphorus transport in plants. However, the biological function of miR399 in Cunninghamia lanceolata is still largely unclear. In this study, the comparison of mature miR399 sequence revealed a high similarity between Arabidopsis thaliana and C. lanceolate, and the pre-miR399 was capable of forming a typical stem-loop hairpin structure. A gene PHOSPHATE 2 (PHO2) was identified as a target of cln-miR399 using 5' rapid amplification of cDNA ends. Furthermore, the relationship between cln-miR399 and PHO2 was further confirmed through a transient co-expression of both genes in Nicotiana benthamiana. To examine the function of miR399 in Arabidopsis, miR399-overexpressing transgenic Arabidopsis thaliana was acquired using Agrobacterium-mediated approach. Real-time PCR showed that the amount of cln-MIR399 transcripts was higher in miR399-overexpressing plants than in wild-type plants, which was accompanied with down-regulation of expression of its target gene AtPHO2. The P content was 1.40 to 1.56-fold higher in the leaves of three transgenic lines than in wild type plants. However, the P content in the roots of the three transgenic lines was 24.5 - 37.2 % less than that in wild type plants. Moreover, the transcriptions of three phosphate transporter genes (PHT1, PHT2, and PHT3) were up-regulated in roots of miR399-overexpressing Arabidopsis plants. Interestingly, the transgenic lines exhibited retarded growth under normal P conditions compared with the wild type. Our findings demonstrate that cln-miR399 may play crucial roles in P transport and plant growth via regulation of its target gene PHO2.