文献类型：期刊文献

中文题名：猪瘟病毒 E2蛋白的原核表达及鉴定

英文题名：Prokaryotic Expression and Identification of E2 Glycoprotein of CSFV

作者：郭东光[1];朱艳平[1];孙国鹏[1];岳峰[1];贾文科[1];王军[1,2];李鹏[1,2];王自浩[1];王选年[1]

机构：[1]新乡学院生命科学与技术系生物技术研究中心;[2]郑州大学

第一机构：新乡学院生命科学技术学院

年份：2014

卷号：35

期号：6

起止页码：45-48

中文期刊名：动物医学进展

外文期刊名：Progress in Veterinary Medicine

收录：北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：河南省基础与前沿技术研究(122300410003;330002;122300410150);国家自然科学基金项目(31201877);河南省教育厅科学技术研究重点项目(13A230837)

语种：中文

中文关键词：猪瘟病毒;E2蛋白;原核表达;鉴定

外文关键词：CSFV;E2 glycoprotein;prokaryotic expression;identification

摘要：以猪瘟病毒(CSFV)E2蛋白为研究对象,通过扩增CSFV C株E2基因,亚克隆至原核表达载体pGEX-6P-1,构建重组原核表达质粒pGEX-6P-1-E2,转化至宿主菌Rostta(DE3)后进行诱导表达。SDSPAGE检测表明与目的蛋白大小一致,其分子质量大小为67.0ku,并且目的蛋白以包涵体形式存在。Western blot表明,重组蛋白能与CSFV兔化高免血清反应并且能被GST标签单抗所识别,表明该融合蛋白正确表达,具有良好的抗原性,为CSFV抗体检测试剂盒的研究奠定了基础。
The E2 protein of CSFV was as the object and E2 gene of CSFV C strain was amplified and then sub-cloned into prokaryotic expression plasmid pGEX-6P-1 vector.The recombinant plasmid named pGEX-6P-1-E2 was transformed into E.coli Rosetta （DE3）and induced by IPTG.A specific expression band with a molecular weight 67.0 ku was detected by SDS-PAGE,and the expressed product was in inclusion body.Western blot indicated that the expressed protein can react with the rabbit antibody to CSFV and can be recognized by the specific monoclonal antibody of GST.The result suggested that the recombinant protein was correctly expressed and has a good antigenicity.This study provides substantial base for re-search of the CSFV antibody detection kit.