文献类型：期刊文献

英文题名：Genetic Diversity of the Wheat Landrace Youzimai from Different Geographic Regions Investigated with Morphological Traits, Seedling Resistance to Powdery Mildew, Gliadin and Microsatellite Markers

作者：Li, X. J.[1,2];Xu, X.[1,3];Yang, X. M.[1];Li, X. Q.[1];Liu, W. H.[1];Gao, A. N.[1];Li, L. H.[1]

第一作者：Li, X. J.

通讯作者：Li, LH[1]

机构：[1]Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China;[2]Henan Inst Sci & Technol, Sch Life Sci & Technol, Xinxiang 453003, Peoples R China;[3]Xinxiang Univ, Dept Life Sci & Technol, Xinxiang 453003, Peoples R China

第一机构：Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

通讯机构：[1]corresponding author), Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China.

年份：2012

卷号：40

期号：1

起止页码：95-106

外文期刊名：CEREAL RESEARCH COMMUNICATIONS

收录：;Scopus(收录号：2-s2.0-84863237698);WOS:【SCI-EXPANDED(收录号:WOS:000300983000011)】；

基金：This work was supported by a grant from the 973 Project of China (No. 200611B100201).

语种：英文

外文关键词：wheat landrace; morphology; powdery mildew; gliadin; microsatellite; genetic diversity

摘要：Youzimai is a widespread wheat landrace and has been used extensively in breeding programs in China. In order to assess the genetic variation between and within Youzimai accessions, samples of 31 landrace accessions of wheat, all called 'Youzimai', were collected from 6 geographic regions in China and evaluated using morphological traits, seedling resistance to powdery mildew, gliadin and microsatellite markers. Typical differences among accessions were observed in morphological characteristics. Forty-five (58.4%) of 77 assayed SSR markers showed polymorphism over the entire collection and total 226 alleles were identified with an average of 5.02 alleles per locus. SSR data indicated that the accessions from Hebei province were the most diverse, as evidenced by greatest number of region-specific alleles and highest diversity index. These accessions, therefore, probably experienced the most substantial morphological and molecular evolution as a result of various natural and anthropomorphic influences. On the other hand, differentiation in gliadin phenotypes was found among seeds within 80.6% of total accessions and average 61.5% of entire collections showed heterogeneous and comprised resistant plants in reaction to powdery mildew, suggesting the presence of a wide diversity within the wheat landrace. By developing an intimate knowledge of the available wheat genotypes, appropriate selections can be made for commercial application in order to conserve and exploit the diversity of the wheat landraces.