文献类型：期刊文献

英文题名：Characterization of quinolone resistance mechanisms in lactic acid bacteria isolated from yogurts in China

作者：Jiang, Xiaobing[1];Yu, Tao[2];Zhou, Danlei[3];Ji, Shengdong[1];Zhou, Chune[1];Shi, Lei[4];Wang, Xuannian[2]

第一作者：Jiang, Xiaobing

通讯作者：Yu, T[1]

机构：[1]Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China;[2]Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453000, Peoples R China;[3]Tongji Univ, Coll Life Sci & Technol, Shanghai 200092, Peoples R China;[4]South China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

第一机构：Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China

通讯机构：[1]corresponding author), Xinxiang Univ, Coll Life Sci & Technol, Xinxiang 453000, Peoples R China.|[1107115]新乡学院生命科学技术学院;[11071]新乡学院;

年份：2016

卷号：66

期号：3

起止页码：1249-1256

外文期刊名：ANNALS OF MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-84963747944);WOS:【SCI-EXPANDED(收录号:WOS:000380710200033)】；

基金：This work was supported by the Key Project of Natural Science of the Education Department of Henan Province, China (15A180006), the Key Scientific and Technological Project of Xinxiang (ZG15007), the Youth Science Foundation of Henan Normal University, and the Doctoral Scientific Research Foundation of Henan Normal University.

语种：英文

外文关键词：Lactic acid bacteria; Quinolone resistance; Yogurt

摘要：Twenty-five strains of ciprofloxacin-resistant lactic acid bacteria (LAB; 14 Lactobacillus spp. and 11 Streptococcus thermophiles spp.) isolated from commercial yogurts in China were analyzed in this study. For each of these strains, amino acid changes associated with quinolone resistance-determining regions (QRDRs) were investigated using PCR-based detection methods. The same methodology was used to identify the presence of plasmid-mediated quinolone resistance (PMQR) genes in LAB. Sequencing analyses and an efflux pump inhibition test (using reserpine) were also performed as part of the analysis. Our results showed that typical mutations corresponding to quinolone resistance were found in the QRDRs of LAB strains. Detected mutations included Ser80Leu in parC, and Ser83Leu and Glu87Asp in gyrA. In addition, a Tyr74Phe substitution in parC, which had not previously been reported to be associated with quinolone resistance, was observed in two Lactobacillus delbrueckii subsp. bulgaricus strains. For each of the tested strains, the presence of the efflux pump inhibitor, reserpine, resulted in a two- to eightfold reduction in the minimum inhibitory concentrations (MICs) of ciprofloxacin. However, PMQR genes were not observed in any of the strains analyzed. Our results suggest that mutations in the QRDRs or efflux pump could be involved in ciprofloxacin resistance, and that a combination of these mechanisms may lead to increased ciprofloxacin resistance in LAB strains.