文献类型：期刊文献

中文题名：禽腺病毒4型ZZ株的分离鉴定及系统进化分析

英文题名：Isolation and Phylogenetic Analysis of Fowl Adenovirus Serotype 4 Isolate ZZ

作者：金前跃[1,2,3];王寅彪[4];柴永笑[1,2,5];卢清侠[1,2,3];李鹏[6];郭振华[1,2,3];邢广旭[1,2,3];邓瑞广[1,2,3];张改平[1,2,3,7]

第一作者：金前跃

机构：[1]河南省农业科学院动物免疫学重点实验室,河南郑州450002;[2]河南省农业科学院中英禽病国际研究中心,河南郑州450002;[3]江苏高校动物重要疾病与人兽共患病防控协同创新中心,江苏扬州225009;[4]新乡医学院公共卫生学院,河南新乡453003;[5]西北农林科技大学动物医学院,陕西杨凌712100;[6]新乡学院生命科学技术学院,河南新乡453003;[7]河南农业大学牧医工程学院,河南郑州450002

第一机构：河南省农业科学院动物免疫学重点实验室,河南郑州450002

年份：2020

卷号：49

期号：11

起止页码：128-133

中文期刊名：河南农业科学

外文期刊名：Journal of Henan Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：国家重点研发计划重点专项(2016YFD0500800);河南省重点研发与推广专项(科技攻关)(202102110100,202102110248);农业部动物疫病观测监测项目(ZX06S1701)。

语种：中文

中文关键词：禽腺病毒;血清4型;分离鉴定;全基因组;系统进化分析

外文关键词：Fowl adenovirus;Serotype 4;Isolation and characterization;Whole genome;Phylogenetic analysis

摘要：为深入研究禽腺病毒4型(FAdV-4)河南流行株的基因组特征和分子进化关系,从患有心包积水综合征的病鸡样品中分离了1株FAdV-4(FAdV-4 ZZ),并对分离毒株进行了全基因组序列分析和分子进化分析。结果表明,FAdV-4 ZZ株可在鸡肝癌细胞上稳定传代并产生明显的细胞病变。与无毒力的FAdV-4 ON1株和FAdV-4 KR5株相比,FAdV-4 ZZ株以及国内流行的CH/JSXZ/2015、JSJ13、SDSX1株均存在ORF19、ORF27、ORF30基因缺失。与国内首次分离的JSJ13株相比,FAdV-4 ZZ株的ORF29序列存在33个碱基缺失。FAdV-4 ZZ株的Hexon基因与国内流行的CH/JSXZ/2015和SDSX1株的核苷酸序列相似性均为100%,与ON1株和KR5株的Hexon基因核苷酸序列相似性分别为98.69%和98.90%。综上,成功分离了FAdV-4 ZZ株,并获得了分离毒株的全基因组序列及其与国内外相关毒株的分子进化关系。
In order to study the genomic characteristics and molecular evolution of FAdV-4 strains circulated in Henan Province,a FAdV-4 strain,named FAdV-4 ZZ,was isolated from HPS-affected chicken and subject to whole genome sequencing and phylogenetic analysis.It was found that FAdV-4 ZZ could stably propagate on LMH cells(chicken hepatoma cell)and produce obvious cytopathic effects.Compared with the non-pathogenic strains FAdV-4 ON1 and FAdV-4 KR5,ORF19,ORF27 and ORF30 genes were absent in FAdV-4 ZZ,CH/JSXZ/2015,JSJ13 and SDSX1 circulated in different areas of China.Compared with the ORF29 of first isolated JSJ13 strain,the ORF29 sequence of FAdV-4 ZZ had 33 base pairs deletion.The identities of Hexon genes between FAdV-4 ZZ and CH/JSXZ/2015,SDSX at the nucleotide level were all 100%,and were 98.69%and 98.90%between FAdV-4 ZZ and strains ON1,KR5,respectively.The current study successfully isolated FAdV-4 ZZ strain,obtained its whole genome sequence and established its phylogenetic relationship with other FAdV strains at home and abroad.