文献类型：期刊文献

中文题名：HPLC法测定紫花地丁中香豆素和黄酮的量

英文题名：Determination of Coumarin and Flavonoids in Viola yedoensis Makino by HPLC

作者：徐小博[1];任敏[1];何松林[2];贾文庆[2];赵坤[3];刘会超[2]

第一作者：徐小博

机构：[1]新乡学院生命科学技术学院,新乡453003;[2]河南科技学院园艺园林学院,新乡453003;[3]河南科技学院动物科技学院,新乡453003

第一机构：新乡学院生命科学技术学院

年份：2019

卷号：0

期号：5

起止页码：312-315

中文期刊名：食品工业

外文期刊名：The Food Industry

收录：北大核心:【北大核心2017】；

基金：河南省科技创新杰出人才计划(174200510004);河南省基础与前沿技术项目(142300410017);河南省科技计划项目(162300410157);河南省科技攻关项目(172102110229)

语种：中文

中文关键词：紫花地丁;香豆素;黄酮;高效液相色谱法

外文关键词：Viola yedoensis Makino;coumarin;flavones;high performance liquid chromatography

摘要：以紫花地丁为材料,建立了同时测定紫花地丁中6种香豆素和黄酮含量的检测方法。结果表明:高效液相色谱(HPLC)选用色谱柱Inertsil ODS-3 C18柱(5μm, 250 mm×4.6 mm);流动相为A (乙腈)和B (15 mmol/L醋酸铵溶液)。梯度洗脱方法, 0~10 min,流动相A 10%~25%;10~20 min,流动相A 25%~60%,紫外检测波长290 nm,柱温30℃,流速1.0 mL/min。6个化合物均在15 min内实现基线分离;线性关系良好(R2>0.998);添加高、中、低浓度的对照品,6个化合物的平均回收率在91.18%~99.92%之间,相对标准偏差均在1.06%~2.14%之间(N=6)。该方法简便、准确、重复性好。对紫花地丁全株和花器官中6种成分含量测定结果表明:全株中秦皮甲素、秦皮乙素、芦丁、木犀草苷、木犀草素、柚皮素的含量分别为2 406, 2 961, 887, 1 371, 382和165 mg/kg,花器官中含量分别为4 345, 5 665, 587, 871,582和465 mg/kg。
A high performance liquid chromatography(HPLC)method for simultaneously determining the content of esculin hydrate,esculetin,rutin,luteolin,luteolin and naringin in Viola yedoensis was established.The chromatographic separation was performed on a Shimadzu LC-20 AT Inertsil ODS-3 C18(5μm,250 mm×4.6 mm).The mobile phase consisted of acetonitrile(A)and 15 mmol/L ammonium acetate in water(B)with linear gradient elution(0-10 min,10%A-25%A,10-20 min,25%A-60%A).The flow rate was 1.0 mL/min.The column temperature was 30℃,and the detection wavelength was 290 nm.Six components were separated to baseline.Each component had a wide linear range and a good linear relationship.Though adding high,medium and low concentrations of reference,the average recoveries were in the range 91.18%to 99.92%with the relative standard deviations ranging from 1.06%to 2.14%(N=6).The developed HPLC method was reproducible,accurate and efficient.The determination results showed that the contents of esculin hydrate,esculetin,rutin,luteolin,luteolin and naringenin in whole plant were 2 406,2 961,887,1 371,382 and 165 mg/kg,and the contents of the six components in flowers were 4 345,5 665,587,871,582 and 465 mg/kg.